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EDUCATIONAL BASIS FOR OPINIONS and PUBLICATIONS

Analytical Chemistry

Physical Chemistry

Organic Chemistry

Minor in English Literature

Bachelor of Science in Chemistry

Evaluation of reports containing results of gas chromatography, mass spectral analysis, e.g. EPA priority pollutants. Analysis of chemical manufacturing protocols and methods to determine by-products. Statistical methods in physical chemistry extend to other disciplines. Communication skills refined by minor in English literature.

Biochemistry

Advanced Topics in Pharmacology and Toxicology

Medical Physiology

Histology

Embryology

Neuro-anatomy

Teratology

Jefferson Medical College

These medical science courses allow the development of opinions that comprise the scientific basis for medical opinions and differential diagnosis. The pharmacology and toxicology expertise is supported by mastering the knowledge of physiological homeostasis. Opinions in the disciplines of neurotoxicology, carcinogenesis, and birth defects are developed from the curriculum of the first year of medical school.

Tissue culture

University of North Carolina

Sterile techniques, normal and malignant cell culture methods with emphasis on cell cycle events. Conducted collagen production and amino acid modification research - thalidomide.

Autoradiography of brain sex hormone receptors

Nuclear protein isolation

Amino acid analysis

Electrophoresis

Chromatography

The specific effects of testosterone and estrogen and their synthetic derivatives in various organ systems were learned. Opinions on the impact of over-dose of these hormones rely upon this knowledge. Knowledge of the biochemistry of mitosis, its control and the loss of control permits development of structure activity relationship models of carcinogens and onco gene theory. Analytical methods learned in the graduate curriculum and in actual laboratory work extends my expertise to laboratory testing and clinical assay utilization.

Baylor College of Medicine Department of Pharmacology - Post Doctoral I

Research relating to the biochemical control mechanisms allows the development of concepts relating to DNA - protein alterations. The discovery of covalent bonding of chemicals to DNA and its relationship to carcinogenic properties by departmental researchers permitted the development of expertise in the chemical basis for carcinogenicity.

Baylor College of Medicine Post Cell Biophysics Department - Post Doctoral II

Faculty and fellows participated in the NIH Super Center Grant for Cardiovascular Research and Demonstration - Michael E. De Bakey, MD, Principal Investigator. From this association, experience in basic research in the biochemistry and physiology of vascular smooth muscle, heart failure, papillary muscle preparations, cholesterol apolipoproteins, calcium release, and dietary effects was obtained.

SCIENTIFIC PUBLICATIONS

Scientific Significance of Each Detailing the Specific Experimental Design Experience,
the Scientific Disciplines Applied and my Educational Underpinnings Supporting the Work

1. Olson, M. O. J., Ezrailson, E. G., Guetzow, K. and Busch, H.
Intranuclear Compartmentalization of Chromatin Proteins: Localization and Phosphorylation of Nuclear and Extranuclear Non-Histone Proteins. Journal of Molecular Biology 97, 611-618, 1975.	Carcinogenic processes studied on a cellular level at the cell nucleus. Detection and elucidation of biochemical processes associated with chromosome function. Top echelon journal.
2. Busch, H., Ballal, R., Busch, R. K., Ezrailson, E. G., Olson, M. O. J., Prestayko, A. W., Taylor, C. W., and Yeoman, L. C.
Chromatin Proteins: Differences in Metabolic, Electrophoretic and Immunological Properties. In: Chromosomal Proteins and their Role in the Regulation of Gene Expression (G. S. Stein, ed.), Academic Press, New York, 1975.	Chromatin Proteins: Differences in Metabolic, Electrophoretic and Immunological Properties. In:Chromosomal Proteins and their Role in the Regulation of Gene Expression (G. S. Stein, ed.), Academic Press, New York, 1975.
3. Ezrailson, E. G., Olson, M. O. J., Guetzow, K. A., and Busch, H.
Phosphorylation of Non-Histone Chromatin Proteins in Normal and Regenerating Liver, Novikoff Hepatoma, and Rat Heart. Federation of European Biochemical Societies Letters 62, 69-73, 1975.	An original report addressing the regulatory processes of various mammalian tissues and cancer cells. A widely read European journal that accepts short papers.
4. Schwartz, A., Entman, M.L., Ezrailson, E. G., Lehotay, D. C., Levey, G.
Possible Cyclic Nucleotide Regulation of a Calcium Pool Mediating Myocardial Contraction. Science 195, 982-990, 1977.	Possible Cyclic Nucleotide Regulation of a Calcium Pool Mediating Myocardial Contraction. Science 195, 982-990, 1977. Demonstration of the role of a second messenger in an isolated heart model. Research addressied this emerging concept in molecular pharmacology. The basis for the actions of adrenaline, serotonin, dopamine, noradrenaline, isoproterenol, and histamine is incorporated in this research. Science is a world-renowned journal found in medical libraries in virtually every country in the world.
5. Schwartz, A., Levey, G. S., Entman, M. L., Ezrailson, E. G., Van Winkle, W. B., Bornet, E. P., and Pitts, B. J. R.
Modulation of Calcium in the Heart. Eighth International Meeting of the International Society of Research in Cardiac Metabolism. In: Recent Advances in Cardiac Structure and Metabolism. University Press, Baltimore, 1976.	Co-authored this review article addressing the research conducted regarding the actions of calcium on the heart muscle. The biophysics, biochemistry, and physiology of the heart and vasulature are the underpinnings for this article. University Press is a prestigious publisher of medical articles.
6. Driscol, D. J., Gillette, P. C., Ezrailson, E. G., and Schwartz, A.
Inotropic Response of the Neonatal Canine Myocardium to Dopamine. Pediatric Research 12, 42-45, 1977.	A research report demonstrating that other agents in the body beside adrenaline promote an increase in the force of the contraction of the heart muscle. Molecular pharmacology in this isolated organ model is the basis for this research. Pediatric Research is a widely read journal by pediatricians and pediatric cardiologist. Dr. Driscol's research was part of the requirements for his residency to become a pediatric cardiologist.
7. Ezrailson, E. G., Potter, J. D., Michael, L. H., and Schwartz, A.
Positive Inotropy Induced by Ouabain and by Isoproterenol: Lack of Correlation with the Phosphorylation of Troponin-I. Journal of Molecular and Cellular Cardiology 9, 693-698, 1977.	Positive Inotropy Induced by Ouabain and by Isoproterenol: Lack of Correlation with the Phosphorylation of Troponin-I. Journal of Molecular and Cellular Cardiology 9, 693-698, 1977. Unique research on the biochemistry and physiology of the contraction of the heart. Demonstration of the biochemical events inside heart muscle cells that lead to the generation of added force of contraction. Cardiologist and research scientists read this journal.
8. Ezrailson, E. G., Garber, A. J., Munson, P. J., Birnbaumer, L., Swartz, T. L., and Entman, M. L.
[125] - Iodopindolol: A New Beta Adrenergic Receptor Probe. Journal of Cyclic Nucleotide Research 7, 13-26, 1981.	This article reports my discovery of a molecular probe used to study adrenaline receptors in every tissue of every animal possessing them. The Journal of Cyclic Nucleotide Research was founded by a Nobel laureate and has published research by other researchers to win this recognition.
9. Ezrailson, E. G., Hanley, H. G., Hartley, C. J., Lewis, R. M., Entman, M. L., and Schwartz, A.
Studies on the Mechanism of an Antibiotic Ionophore. RO2- 2985(X537A) in the Conscious Chronically Instrumented Dog - Involvement of a Prostaglandin Synthetic Pathway. Cardiovascular Research 16, 670-674, 1982.	The results of this research further established the biochemical basis and physiology of kidney failure after hemorrhagic shock. The role of calcium and the "tissue messenger" - Prostaglandin was discovered. The basic understanding of kidney function, arterial smooth muscle physiology, enzymes producing tissue messengers, and the biochemical basis for the physiological response to severe blood loss are the underpinnings for this research.
10. Ezrailson, E. G., Entman, M. L., and Garber, A. J.
Adrenergic and Serotonergic Regulation of Skeletal Muscle Metabolism in the Rat: The Effects of Adrenergic and Serotonergic Antagonists on the Regulation of Muscle Amino Acid Release, Glycogenolysis, and Cyclic Nucleotide Levels. Journal of Biological Chemistry 258, 12494-12498, 1983.	A report on research on the anabolic effect of adrenaline and serotonin on muscle. Links were found to an interior messenger for these two neurotransmitters. The basis and expertise required to design and execute the experiments for this research was an extensive knowledge of enzyme kinetics and biochemical metabolism. The Journal of Biological Chemistry has one of the most stringent review processes. Very few of the articles submitted are published.
11. Morette-Rojas, I., Ezrailson, E. G., Entman, M. L., Birnbaumer, L., and Garber, A. J.
Serotonergic and Adrenergic Regulation of Skeletal Muscle Metaboism in the Rat: the Use of [125] Iodolysergic Acid Diethylamide and [125] Iodopindololol as Probes of Sarcolemmal Function and Specificity. Journal of Biological Chemistry 258, 12499-12508, 1983.	The adrenaline and serotonin receptors were studied as the mediators of the anabolic phenomenon reported in the paper above. This publication reports the development of the serotonin receptor probe (radioactive) [125] iodolysergic acid diethylamide. Nuclear Nagnetic Resonance was used to identify the exact site where the isotope is incorporated.The Journal of Biological Chemistry has one of the most stringent review processes. Very few of the articles submitted are published.
12. Wolf, L.E., Lappé, M., Peterson, R.D., and Ezrailson, E. G.
Human immune response to polydimethylsiloxane (silicone): screening studies in a breast implant population. FASEB Journal 7, 1265-1268, 1993.	This research project was a blind study. (The condition of the implants was not known to the group that determined the antibody levels and the physicians removing the implants did not know the result of the antibody tests.) Using this sensitive assay, silicone reactive antibody levels are determined and related to the condition of the implant and a control group. Rupture and leakage can be detected by this assay. Intact implant recipients had significant levels of antisilicone antibody levels compared to women with no implants. Data were analyzed by an independent group that reported the results. FASEB Journal is a publication of eight basic medical science societies.
13. Lewy, R.I. and Ezrailson, E. G.
Laboratory studies in breast implant patients: ANA positively, gammaglobin levels, and other autoantibodies. Current Topics in Microbiology and Immunology 210, 337-353, 1996.	Analysis of antinuclear antigen (an autoimmune response) levels revealed women with silicone breast implant have significantly increased levels. The results support the possibility that the silicone implants cause various disease conditions. The result were presented at an NIH seminar and published in one of the Institutes journals.

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